Intimate partner violence against women during covid-19: A population-based study in Vitória, state of Espírito Santo, Brazil.

BACKGROUND: Violence against women has a negative impact on multiple dimensions of women's health. During the Covid-19 pandemic, intimate partner violence against women has continued, and in some contexts has intensified. The aim of this study was to identify the prevalence of intimate partner violence against women during covid-19 pandemic and its association with socioeconomic, behavioral, and life-experience factors. METHODS AND FINDINGS: Cross-sectional, population-based study conducted in the municipality of Vitória, state of Espírito Santo, from January to May 2022, where 1,086 women aged 18 years and over were interviewed. The World Health Organization (WHO) instrument on violence against women was used to screen outcomes. The prevalence of violence during the pandemic (psychological, physical, and sexual) and bivariate analysis with sociodemographic, behavioral, family, and life history characteristics of women were estimated. The multivariate analysis was carried out for each type of violence, the Poisson regression model was performed with an estimate of robust variance, inserting the variables of interest with (p<0.20). Those with p<0.05 remained in the adjusted model. RESULTS: The prevalence of violence psychological against women perpetrated by an intimate partner during the pandemic was the most frequent (20.2%), followed by physical (9.0%) and sexual violence (6.5%). Women with less schooling and who were single had a higher prevalence of physical and psychological violence, as did those with a history of sexual abuse in childhood and whose mothers had been beaten by their intimate partners. Sexual violence was more prevalent among non-white, with up to eight years of schooling, whose mothers had a history of intimate partner violence, and who consumed alcohol during four days or more (p<0.01). CONCLUSION: Psychological, physical, and sexual violence perpetrated by the intimate partner during the pandemic presented high magnitude among women living in Vitória. Sociodemographic, behavioral factors, and personal and maternal experiences of violence were associated with the phenomenon.

Advanced lithium disilicate: A comparative evaluation of translucency and fatigue failure load to other ceramics for monolithic restorations.

The aim of this in vitro study was to evaluate the surface roughness, translucency, fatigue failure load (FFL), and number of cycles for fatigue failure (CFF) of a recently released lithia-based material called advanced lithium disilicate and three other ceramics indicated for monolithic restorations. First, ALD (advanced lithium disilicate, CEREC Tessera, Dentsply Sirona), LD (lithium disilicate, IPS e. max CAD, Ivoclar), LS (lithium silicate-disilicate, Suprinity, Vita Zahnfabrik), and 4Y-PSZ (Yttria-stabilized zirconia, IPS e.max ZirCAD MT, Ivoclar) discs (n = 15, Ø = 10 mm and thickness = 1.0 mm) were fabricated from CAD/CAM blocks/discs, A2 shade. The discs were sintered/crystallized and subsequently analyzed by a rugosimeter (Mitutoyo SJ-410) to determine Ra and Rz surface roughness parameters. Next, they were evaluated to determine the translucency parameter (TP) using a bench-top spectrophotometer (SP60, EX-Rite). The discs were subsequently cemented to glass fiber epoxy resin discs, and the specimens were tested under cyclic loading (Step-test), immersed in distilled water at a frequency of 20 Hz, with an initial cyclic load of 200 N for 5,000 cycles and increments of 50 N every 10,000 cycles until failure. Fatigue failure load (FFL) and number of cycles for fatigue failure (CFF) were recorded for subsequent Kaplan Meier analysis, with post-hoc Mantel-Cox and Weibull analysis (α = 0.05). Complementary fractographic, topographic and energy dispersive spectroscopy analyses (EDS) were performed. 4Y-PSZ showed higher survival (p < 0.05), with higher FFL and CFF (1077 N; 180,333 cycles), followed by LD (980 N; 161,000 cycles), LS (937 N; 152,333 cycles) and ALD (910 N; 147,000 cycles). No differences were observed between the tested groups regarding Weibull modulus. ALD presented TP (28.14) equal to DL (28.27) and higher than LS (25.51). All lithia-based materials had higher translucency than 4Y-PSZ (TP = 8.62) (p < 0.05). ALD appears to have a similar elemental composition to LD and LSD for oxygen and silicon. ALD and LSD have a similar zirconium content. Fractures originated on the cemented surface of the ceramic discs. Lithia-based ceramics showed lower surface roughness, with ALD (Ra = 0.04 µm; Rz = 0.66 µm) showing the lowest values (p < 0.05). Despite showing lower FFL when compared to LD and 4Y-PSZ, ALD has compatible translucency and mechanical fatigue performance with its indication for fabricating monolithic, anterior and posterior adhesively cemented single-unit restorations. However, further studies are needed to substantiate its clinical performance.

Causes of fetal death in the Flemish cattle herd in Brazil.

Background and Aim: Flemish cattle in Brazil are on the brink of extinction and are found only in one herd in Lages, Santa Catarina State. This study aimed to uncover the reasons for the recurring abortions in the Flemish cattle herd. Materials and Methods: Seventeen Flemish fetuses underwent postmortem examinations, with samples collected for histopathology and microbiology culture tests, polymerase chain reaction (PCR) test for Neospora caninum, and reverse transcription-PCR (RT-PCR) test for bovine viral diarrhea virus (BVDV) from 2015 to 2020. Results: Of the 17 fetuses, N. caninum was the most common diagnosis and was found in 88% (15/17). One fetus (5.8%) had a coinfection with N. caninum and Citrobacter amalonaticus, leading to fibrinonecrotic pericarditis. All fetuses tested negative for BVDV by RT-PCR. Of the 107 dams tested by indirect immunofluorescence assay, 26 (25.2%) were anti-N. caninum seropositive, with 17 (65.4%) aborting and 5 (19.2%) having estrus repetition. Reverse transcription-PCR results showed that 9 (8.4%) of the serum samples collected from dams tested positive, which tested follow-up test 3 months later, indicating a BVDV transient infection. The factors that contributed to neosporosis included dogs' access to pastures and improper disposal of fetal remains, which made it easier for dogs to consume them. Conclusion: This study warns the occurrence of N. caninum as a cause of reproductive disorders that can lead to abortion in the studied Flemish cattle herd.

Violência autoprovocada no Espírito Santo: uma análise dos casos notificados / Self-inflicted violence in Espírito Santo: an analysis of reported cases / Violencia autoinfligida en Espírito Santo: un análisis de los casos notificados

Objetivo: identificar a frequência de casos notificados de violência autoprovocada no Espírito Santo entre os anos de 2011 a 2018 e os fatores associados. Método: trata-se de um estudo transversal que utilizou todos os casos notificados de violência autoprovocada registrados no Sistema de Informação de Agravos de Notificação entre 2011 e 2018 no estado do Espírito Santo. A análise multivariada foi realizada pela Regressão de Poisson e o estudo foi aprovado pelo Comitê de Ética em Pesquisa. Resultados: a prevalência da violência autoprovocada foi de 25,1% (IC95%: 24,6-25,5). O grupo de 10 a 19 anos de idade teve uma prevalência cerca de 19 vezes maior de notificação de violência autoprovocada, assim como, pessoas de raça/cor branca (RP: 1,26) e com deficiência/transtornos (RP: 2,85) apresentaram maior frequência desse agravo. Foi observado maior prevalência entre aqueles sem suspeita de uso de álcool (RP: 2,36), com a ocorrência cerca de 4 vezes maior na residência, e, um aumento em cerca de 50% dos casos sem caráter de repetição. Conclusão: a violência autoprovocada no Espírito Santo se apresentou elevada no período estudado e esteve associada a características da vítima e do evento. Profissionais de saúde são fundamentais no processo de rastreio desse agravo e consequentemente em traçar estratégias de prevenção e proteção das vítimas.(AU)

Objective: to analyze the prevalence of reported cases of self-inflicted violence and associated factors in Espírito Santo between 2011 and 2018.Method: this is a cross-sectional study that used all reported cases of self-inflicted violence registered in the Sistema de Informação de Agravos de Notificação between 2011 and 2018, in the state of Espírito Santo. Multivariate analysis was performed using Poisson Regression.Results: the prevalence of self-inflicted violence was 25.1%. The 10 to 19-year-old group had a prevalence of approximately 19 times greater self-inflicted violence report, as did people of white race/color (PR: 1.26) and people with disabilities/disorders (PR: 2.85) showed a higher frequency of this problem. A higher prevalence was observed among those without suspected alcohol use (PR: 2.36), with an occurrence approximately four times higher in the home, and an increase in approximately 50% of non-repeating cases.Conclusion: reports of self-inflicted violence in Espírito Santo were high during the period studied and were associated with characteristics of the victim and the event. Health professionals are fundamental in the process of tracking this problem and consequently in the process of designing prevention and protection strategies for victims.(AU)

Objetivo: analizar la prevalencia de casos notificados de violencia autoinfligida en Espírito Santo entre los años 2011 y 2018, así como los factores asociados. Método: se trata de un estudio transversal que utilizó todos los casos notificados de violencia autoinfligida registrados en el Sistema de Información de Agravios de Notificación entre 2011 y 2018 en el estado de Espírito Santo. El análisis multivariado se llevó a cabo mediante la Regresión de Poisson. Resultados: la prevalencia de la violencia autoinfligida fue del 25,1%. El grupo de 10 a 19 años de edad presentó una prevalencia aproximadamente 19 veces mayor de notificación de violencia autoinfligida. Asimismo, las personas de raza/color blanco (RP: 1,26) y con discapacidad/trastornos (RP: 2,85) mostraron una mayor frecuencia de este agravio. Se observó una mayor prevalencia entre aquellos sin sospecha de uso de alcohol (RP: 2,36), con una ocurrencia aproximadamente cuatro veces mayor en la residencia, y un aumento de alrededor del 50% en los casos sin carácter repetitivo. Conclusión: las notificaciones de violencia autoinfligida en Espírito Santo fueron elevadas en el período estudiado y se asociaron con características de la víctima y del evento. Los profesionales de la salud desempeñan un papel fundamental en el proceso de detección de este agravio y, consecuentemente, en el diseño de estrategias de prevención y protección de las víctimas.(AU)

Clinical and Antinociceptive Effects of Distal Inferior Alveolar Nerve Block in Ponies With Tramadol 5% or Lidocaine 2.

This study aimed to compare the antinociceptive effects of tramadol 5% and lidocaine 2% on mental nerve block in horses of the Brazilian Pony breed. Eight adult non-pregnant mares were used in this study. The ponies were tranquilized with acepromazine (5 µg kg-1, IV), and the infiltration of the mental foramen was performed in Treatment 1- tramadol 5% (T, 150 mg) or Treatment 2- lidocaine 2% (L, 60 mg), both at a total dose of 3 ml in each foramen. Heart rate (HR), respiratory rate (RR), systolic arterial pressure (SAP), diastolic arterial pressure (DAP), mean arterial pressure (MAP), rectal temperature (RT), and formation of skin lesions (SL) were evaluated. Evaluation of nociception of the outer lip (OL), inner lip (IL), and gingiva (GG) were performed using an electronic von Frey device with the evaluation of the ponies' reactions to each stimulus. From these reactions, we determined latency time (LT) and duration of antinociception (DAN). Analysis of variance with 16 observations was performed for HR, RR, SAP, DAP, MAP, LT and DAN. Data were expressed as mean ± standard deviation and the means were compared by the SNK and Student's t-test (P< 0.05). Changes in HR, RR, SAP, DAP, and MAP between evaluation times were associated with the effects of acepromazine (P>0.05). No formation of skin lesions was observed. Latency time did not differ between treatments (P>0.05). Both lidocaine 2% and tramadol 5% produced an antinociceptive effect. We conclude that the duration of the antinociceptive effect of tramadol 5% is longer than that of lidocaine 2%.

Análise dos casos de violência interpessoal contra mulheres / Análisis de casos de violencia interpersonal contra mujeres / Analysis of cases of interpersonal violence against women

Resumo Objetivo Analisar os casos notificados de violência interpessoal contra as mulheres no estado do Espírito Santo. Métodos Estudo analítico, do tipo transversal, em que foram analisados os casos notificados de violência contra a mulher no período de 2011 a 2018 no Espírito Santo. Os dados que foram analisados neste estudo são resultantes das Fichas de Notificação/Investigação de Violência Interpessoal e Autoprovocada provenientes do Sistema de Informação de Agravos de Notificação (SINAN). As análises estatísticas foram realizadas por meio do teste de Qui-quadrado e regressão de Poisson, utilizando o software Stata 14.1, sendo considerados significativos valores de p menores que 0,05. Resultados A notificação de violência interpessoal no sexo feminino representou frequência de 74,9% (n=20.449; IC95%: 74,4-75,4). Foi observado que crianças e idosas apresentaram 35% mais prevalência desse agravo comparada aos adolescentes. Há maior frequência desse fenômeno entre pessoas de cor preta / parda (RP: 1,07; IC95%:1,05-1,08) comparada àquelas de cor branca. Quanto às características do agressor verifica-se maior prevalência na faixa etária de 25 anos e mais (RP: 1,09; IC95%: 1,06-1,11), do sexo masculino (RP: 3,02; IC95%: 2,88-3,17) e sob suspeita de uso de álcool no momento da agressão (IC95%: 1,05-1,08). Em relação ao evento, nota-se uma frequência 1,28 vezes maior em via pública. Conclusão Fatores sociodemográficos, comportamentais e experiências pessoais de violência influenciam a ocorrência do fenômeno.

Resumen Objetivo Analizar los casos notificados de violencia interpersonal contra mujeres en el estado de Espírito Santo. Métodos Estudio analítico, tipo transversal, en el que se analizaron los casos notificados de violencia contra la mujer en el período de 2011 a 2018 en Espírito Santo. Los datos que fueron analizados en este estudio son resultantes de las Fichas de Notificación/Investigación de Violencia Interpersonal y Autoprovocada provenientes del Sistema de Información de Agravios de Notificación (SINAN). Los análisis estadísticos se realizaron mediante la prueba χ2 de Pearson y regresión de Poisson, con el uso del software Stata 14.1, donde se consideraron significativos valores de p menores que 0,05. Resultados La notificación de violencia interpersonal en el sexo femenino representó una frecuencia de 74,9 % (n=20.449; IC95 %: 74,4-75,4). Se observó que niñas y adultas mayores presentan un 35 % más de prevalencia de este agravio, comparadas con adolescentes. Existe una mayor frecuencia de este fenómeno en personas de color negro / pardo (RP: 1,07; IC95 %:1,05-1,08), comparadas con las de color blanco. Respecto a las características del agresor, se verificó mayor prevalencia en el grupo de edad de 25 años o más (RP: 1,09; IC95 %: 1,06-1,11), de sexo masculino (RP: 3,02; IC95 %: 2,88-3,17) y con sospecha de uso de alcohol en el momento de la agresión (IC95 %: 1,05-1,08). Con relación al evento, se observa una frecuencia 1,28 veces mayor en la vía pública. Conclusión Factores sociodemográficos, de comportamiento y experiencias personales de violencia influyen para que suceda este fenómeno

Abstract Objective To analyze the reported cases of interpersonal violence against women in the state of Espírito Santo. Methods This is an analytical, cross-sectional study, which analyzed the reported cases of violence against women from 2011 to 2018 in Espírito Santo. The data that were analyzed in this study are the result of the Reporting Forms/Investigation of Interpersonal and Self-inflicted Violence from the Reporting Diseases Information System (SINAN). Statistical analyzes were performed using the chi-square test and Poisson regression, using Stata 14.1, with p-values lower than 0.05 being considered significant. Results Interpersonal violence reporting in females represented a frequency of 74.9% (n=20,449; 95%CI: 74.4-75.4). It was observed that children and older adults had a 35% higher prevalence of this condition compared to adolescents. There is a higher frequency of this phenomenon among black/brown people (PR: 1.07; 95%CI: 1.05-1.08) compared to white people. As for aggressor characteristics, there is a higher prevalence in the age group of 25 years and older (PR: 1.09; 95%CI: 1.06-1.11), male (PR: 3.02; 95%CI: 2.88-3.17) and under suspicion of alcohol consumption at the time of aggression (95%CI: 1.05-1.08). Regarding the event, there is a frequency 1.28 times higher on public thoroughfare. Conclusion Sociodemographic, behavioral factors and personal experiences of violence influence its occurrence.

Violência física contra o sexo feminino no espírito santo / Violencia física contra la mujer en espírito santo-brasil / Physical violence against women in espírito santo

RESUMO Objetivo: identificar a frequência da notificação de violência física contra o sexo feminino no estado do Espírito Santo, no período de 2011 a 2018, e sua associação com as características da vítima, do agressor e da ocorrência. Método: estudo transversal, com análise dos casos de violência física contra mulheres notificadas no Sistema de Informação de Agravos de Notificação em 2011-2018. As associações foram testadas pelo teste qui-quadrado de Pearson e regressão de Poisson. Resultados: a notificação de violência física contra meninas e mulheres representou uma frequência de 54,1%. O grupo feminino de 20 a 59 anos tem cerca de 4,3 vezes mais prevalência de ser vítima de violência física quando comparado ao grupo de 0-9 anos. As mulheres pretas/pardas têm 1,06 vezes mais frequência de notificação de violência física. A maior parte das ocorrências do evento foi observado entre as sem deficiência/transtorno e residentes na zona urbana/periurbana. A violência física contra mulheres foi 1,71 vezes mais praticada por um conhecido, e com suspeita de uso de álcool (RP:1,07). Conclusão: a violência física contra o sexo feminino foi elevada e apresenta associação com determinadas características da vítima do agressor e da ocorrência, tais achados podem nortear as tomadas de decisões no enfrentamento a violência.

RESUMEN Objetivo: identificar la frecuencia de notificación de violencia física contra el sexo femenino en el estado del Espírito Santo-Brasil, en el período de 2011 a 2018, y su asociación con las características de la víctima, del agresor y de la ocurrencia. Método: estudio transversal, con análisis de los casos de violencia física contra mujeres notificados en el Sistema de Información de Agravios de Notificación en 2011-2018. Las asociaciones fueron comprobadas por la prueba Chi-cuadrado de Pearson y regresión de Poisson. Resultados: la notificación de violencia física contra niñas y mujeres representó una frecuencia de 54,1%. El grupo femenino de 20 a 59 años tiene cerca de 4,3 veces más prevalencia de ser víctima de violencia física cuando comparado al grupo de 0-9 años. Las mujeres negras/pardas tienen 1,06 veces más frecuencia de notificación de violencia física. La mayor parte de las ocurrencias del evento fue observada entre las sin discapacidad/trastorno y residentes en la zona urbana/periurbana. La violencia física contra mujeres fue 1,71 veces más practicada por un conocido, y con sospecha de uso de alcohol (RP:1,07). Conclusión: la violencia física contra el sexo femenino fue elevada y presenta asociación con determinadas características de la víctima del agresor y de la ocurrencia, tales hallazgos pueden guiar las tomas de decisiones en el enfrentamiento a la violencia.

ABSTRACT Objective: to identify the frequency of notification of physical violence against women in the state of Espírito Santo, from 2011 to 2018, and its association with the characteristics of the victim, the aggressor and the occurrence. Method: cross-sectional study with analysis of cases of physical violence against women reported in the Notifiable Diseases Information System in 2011-2018. The associations were tested by Pearson's chi-square test and Poisson regression. Results: the notification of physical violence against girls and women represented a frequency of 54.1%. The female group aged 20 to 59 years has about 4.3 times more prevalence of being a victim of physical violence when compared to the group aged 0-9 years. Black/brown women have 1.06 times more frequency of notification of physical violence. Most of the occurrences of the event were observed among those without disability/disorder and residents in the urban/periurban area. Physical violence against women was 1.71 times more practiced by an acquaintance, and with suspected alcohol use (PR:1.07). Conclusion: physical violence against women was high and presents association with certain characteristics of the victim of the aggressor and the occurrence, such findings can guide decision-making in coping with violence.

The possible benefits of vitamin D in COVID-19.

Molecular studies have demonstrated the importance of the exacerbated immune response to SARS-CoV-2 infection, called the cytokine storm, in more severe COVID-19. The pathophysiology is complex and involves several homeostatic factors; among them, a deficit of vitamin D draws attention because of its high frequency in the population. Some evidence suggests that people with low serum vitamin D levels have worse outcomes, often requiring intensive care. This review analyzed the studies available in the global literature addressing the benefits of vitamin D in COVID-19, relating serum levels to the severity of the disease, and indicating vitamin D as a possible prophylactic and therapy in infection.

Painless and painful synovitis have similar ultrasound and clinical outcomes: one-year cohort study in longstanding rheumatoid arthritis women patients.

OBJECTIVES: We aimed to compare the painless synovitis evolution with painful synovitis, based on bone erosion by ultrasonography over a year in women with longstanding rheumatoid arthritis. Ultrasound inflammatory measurements and radiographic, functional and clinical findings were also compared between groups at the end of the same follow-up. METHODS: A prospective cohort study was rolled out, involving 60 women with RA, divided into two groups: painless and painful, with 30 patients in each group. The wrist and MCPs joints were assessed by ultrasound and plain x-ray, initially and after 12 months (T0 and T12). There was also a clinical assessment (activity scores, functional tests, disease and treatment progression variables) at 6 and 12 months. RESULTS: Patients' average age was 58.0±12.8 and average length of disease 16.4±9.8 years. Initially, the demographic characteristics were similar between groups, however, the painful group had worse clinical and functional scores. There were no statistically significant differences in the majority of US bone erosions and US inflammatory measurements, nor in radiographic progression variables between the groups. Over one year, pinch strength test and DAS 28 remained worse in the painful group (p<0.05). Clinical worsening variables and change of treatment evolved similarly between the groups, on T6 and T12. CONCLUSIONS: According to the study, the painless group progressed similarly to the painful one over a year, as regards bone erosion, ultrasound inflammatory measurements, radiographic findings, clinical worsening and change of treatment in female longstanding RA patients.

Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses / Proteínas de fase aguda do lavado broncoalveolar de equinos hígidos

Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis. Ceruloplasmin, transferrin, albumin, α1-antitripsin, immunoglobulin G heavy, immunoglobulin G light, immunoglobulin A, haptoglobin, acidic glycoprotein, and P23 were measured in BALF from 30 horses without inflammatory disease by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In serum, the same proteins were identified except for α1-antitrypsin. In conclusion, this study demonstrated that polyacrylamide gel electrophoresis (SDS-PAGE) can be used for the determination of acute-phase proteins in BALF samples from horses. In healthy horses, the values are very low, but they can be compared with reference values to assist in the diagnosis of animals with respiratory diseases.(AU)

O líquido obtido através da lavagem broncoalveolar (LBA) foi analisado para obter informações sobre as proteínas da fase aguda. Ceruloplasmina, transferrina, albumina, α1-antitripsina, imunoglobulina G pesada, imunoglobulina G leve, imunoglobulina A, haptoglobina, glicoproteína ácida e P23 foram medidas nos LBA de 30 cavalos sem doença inflamatória por eletroforese em gel de poliacrilamida com dodecilsulfato de sódio (SDS-PAGE). No soro, as mesmas proteínas foram identificadas, exceto a α1-antitripsina. Em conclusão, este estudo demonstra que a eletroforese em gel de poliacrilamida (SDS-PAGE) pode ser usada para a determinação de proteínas de fase aguda em amostras de LBA em cavalos. Em cavalos saudáveis, os valores são muito baixos, no entanto, podem ser comparados e auxiliar no diagnóstico de animais com doenças respiratórias.(AU)

ELISA of amyloid A in paired bronchoalveolar lavage fluid and serum samples of healthy horses / Detecção de amilóide A no lavado broncoalveolar de equinos hígidos

Pulmonary disorders are common in horses, and treatment efficiency depends on an adequate diagnosis. Amyloid A is the most sensitive indicator of pathology in horses. The objective of this study was to establish the concentration of amyloid A of bronchoalveolar lavage fluid (BALF) in healthy horses. Health condition of horses was considered normal based on physical examination, complete blood count, biochemical parameters, and BALF cytology. Blood and BALF were collected from thirty adult female horses. Amyloid A concentrations in serum and BALF were measured using commercial ELISA tests. Amyloid A was detected in serum (mean ± SD = 3.71±2.51) and BALF (mean ± SD = 0.000745±0.000785) of all horses. In conclusion, SAA can also be measured in bronchoalveolar fluid, affording early detection of respiratory infections or inflammatory conditions.(AU)

Distúrbios pulmonares são comuns nos cavalos e a eficiência do tratamento depende de um diagnóstico adequado e precoce. A amilóide A é um biomarcador sensível na deteccção de patologias inflamatórias e infecciososa em cavalos. O objetivo deste estudo foi estabelecer a concentração de amilóide A no líquido broncoalveolar (LBA) em cavalos saudáveis. Os cavalos foram considerados saudaveis baseado nos achados de normalidade do exame físico, hemograma, parâmetros bioquímicos e citologia do LBA. Sangue e LBA foram coletados de 30 fêmeas equinas adultas. Os níveis de Amilóide A no soro e no LBA foram mensurados por meio do teste de ELISA. A amilóide A foi detectada no soro (média ± DP = 3,71±2,51) e no LBA (média ± DP = 0,000745±0,000785) de todos os animais. Conclui-se que a amilóide A também pode ser mensurada no LBA, auxiliando no diagnóstico precoce de processos inflamatórios e infecciosos pulmonares.(AU)

Causes of death of beef cattle raised in feedlots / Causas de morte de bovinos de corte criados em confinamentos

The causes of death of cattle kept in pre-export feedlots (PEFs) and in feedlot for finishing for slaughter are described. Two studies were conducted: a retrospective study of mortality cases in feedlots from 2000 to 2017 registered at the "Laboratório Regional de Diagnóstico" of the "Faculdade de Veterinária" of the "Universidade Federal de Pelotas"; and a prospective study from January 2018 to August 2019, following up 22 feedlots for finishing and six PEFs for the export of live cattle. From January 2000 to August 2019 samples of 150 cases of diseases that affected feedlot cattle were received from 22 feedlots for finishing and 115 of the six PEFs followed. Mortality considering all diagnosed diseases was significantly higher in feedlots for finishing (p<0.05), than in PEFs for the export of live cattle, of 1% and 0.12%, respectively. Diseases of the digestive system were the most important causes, of death in feedlots regardless of its purpose. Acidosis presented the highest mortality rates both in feedlot for finishing (3.33%) as in PEFs for export (0.95%). In all cases the disease occurred due to failure in the adaptation of animals to the ingestion of concentrated foods. Bovine tick fever and pneumonia presented mortality rates of 0.13% and 0.09%, respectively in PEFs. In the feedlot for finishing seneciosis was the second cause of death due to cattle coming from areas with high infestation by the plant. In the present study, it was possible to identify the main diseases that occur in cattle feedlots for finishing or for the export of live animals in the southern region of Rio Grande do Sul. These diseases are known in other systems of cattle breeding and can be prevented or controlled through management, chemoprophylaxis or vaccination, minimizing losses due to mortality.(AU)

Descrevem-se as causas de morte de bovinos mantidos confinados em estabelecimentos pré-embarque (EPEs) para exportação de animais vivos e em estabelecimentos de terminação para abate. Foram realizados dois estudos: um retrospectivo dos casos de mortalidade em confinamentos de 2000 a 2017 registrados no Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas; e um estudo prospectivo de janeiro de 2018 a agosto de 2019, acompanhando-se 22 confinamentos de terminação e seis EPEs para exportação de bovinos vivos. No total, de janeiro de 2000 a agosto de 2019 foram recebidos no LRD-UFPel amostras de 150 casos de enfermidades que afetaram bovinos confinados, 35 provenientes de 22 confinamentos de terminação e 115 dos seis EPEs acompanhados. A mortalidade considerando-se todas as enfermidades diagnosticadas foi significativamente maior nos confinamentos para terminação (p<0,05), do que nos EPEs para exportação de bovinos vivos, de 1% e 0,12%, respectivamente. As doenças do sistema digestivo foram as causas de morte mais importante nos confinamentos, independente da finalidade. Acidose apresentou as maiores taxas de mortalidade tanto nos confinamentos para terminação (3,33%) como nos EPEs para exportação (0,95%). Em todos os casos a doença ocorreu devido a falha na adaptação dos animais à ingestão de alimentos concentrados. Tristeza parasitária bovina e pneumonias apresentaram taxas de mortalidade de 0,13% e 0,09, respectivamente em EPEs de exportação. Nos confinamentos para terminação a seneciose foi a segunda causa de morte devido aos bovinos serem provenientes de áreas com alta infestação pela planta. Este estudo permitiu identificar as principais enfermidades diagnosticadas em confinamentos para terminação de bovinos ou para exportação de bovinos vivos na região Sul do Rio Grande do Sul. Observou-se que são doenças que ocorrem em outros sistemas de criação de bovinos e que podem ser prevenidas ou controladas por meio de manejo, quimioprofilaxia ou vacinação, minimizando prejuízos por mortalidade de animais.(AU)

Influence of Exercise, Age, Body weight, and Growth on the Development of Tarsal Osteoarthritis in Young Mangalarga Marchador Horses.

In this study, the effect of early exercise, age, body weight (BW), and growth on the articular cartilage and subchondral bones of the tarsocrural joints was evaluated in 40 young Mangalarga Marchador horses allowed free choice exercise in pasture. Twenty of the horses had additional controlled exercise 3 days per week from a mean age of 30 ± 20 days until 36 months. The training program consisted of an increasing number of 15-minute gallop sprints in an oval paddock with a concrete floor covered by a thick layer of sand. BW, withers height (WH), and neck circumference were measured and body condition scores, cresty neck scores, and obesity index were rated. For each tarsus and foal, 5 standard radiographic projections were evaluated. All evaluations were performed at time point 1 (18 months of age) and time point 2 (36 months of age). Radiographic changes suggestive of tarsal osteoarthritis were observed in two male foals of the trained group at time point 2 (10% of 20). No horses from the untrained group developed OA. Training of the foals did not result in alterations of the morphometric parameters evaluated. However, significant differences were found between time point 1 and 2 in trained and untrained animals. At time point 2, the animals presented greater weights and WHs than at time point 1. We conclude that specific levels of physical activity during initial development do not increase the prevalence of osteoarthritic injury in Mangalarga Marchador foals.

Use of detention basin for flood mitigation and urban requalification in Mesquita, Brazil.

Unplanned urbanization is one of the main factors responsible for worsening flood-related problems in cities, increasing the frequency of flooding and flooding depths, consequently degrading both the natural and built environment. Considering this, the use of engineering techniques that reduce runoff and promote urban requalification are an efficient option for managing rainwater. This paper presents a case study of a flood control project using a storm water detention pond, designed to allow multiple uses of an urban space. The operation of the system is evaluated by an urban flow-cell model, known as MODCEL. This application seeks the best configuration for the layout of 'Celso Peçanha' Detention Basin, considering the local restrictions imposed by the City of Mesquita - Brazil, and optimized to damp storm flows resulting from rainfall events with return periods up to 50 years. The solution proposed considers the possibility of social urban space uses in flood control projects, revitalizing degraded areas and giving them multiple functions.

Leukoencephalomalacia in horses associated with immature corn consumption / Surto de leucoencefalomalacia em equinos associado ao consumo de milho verde

ABSTRACT: Horse leukoencephalomalacia (ELEM) is a disease caused by the ingestion of mycotoxins (fumonisins) produced by fungi of the genus Fusarium that infect corn and/or its byproducts. This disease has been described by ingestion of mature corn with humidity above 15% at temperatures below 20°C. The aim of this paper was to report an outbreak of leukoencephalomalacia in horses fed with immature corn. Two horses out of three showed neurological signs approximately seven days after eating immature corn in its reproductive phase (R2, milky grains). Corn was harvested and administered directly to the animals, with no storage. Deaths occurred approximately 24 hours after the onset of clinical signs. Grossly, there were multifocal dark red to brown areas in the white matter of the telencephalon and hyppocampus and thalamus. Histologically, there was edema and hemorrhage in several areas of the telencephalon white matter, which corresponded to dark red to brown areas observed in the macroscopy. There was also foci of malacia with presence of reactive astrocytes with abundant eosinophilic cytoplasm and inflammatory cells. Diffuse capillary wall degeneration and endothelial cell swelling were also observed. Two ppm of fumonisin were detected by immunoaffinity column method (VICAM) in the immature corn sample. The water activity in this cereal, when the grain is still milky, is 0.98 and can predispose it to growth of mycotoxin-producing fungi. In the present case, fumonisin was found in milky grains in the beginning of the reproductive phase (R2), which suggested that even immature corn may be infected by Fusarium spp. and should not be administered to horses.

RESUMO: A leucoencefalomalácia dos equinos (ELEM) é uma doença causada pela ingestão de micotoxinas (fumonisinas) produzidas por fungos do gênero Fusarium que infectam o milho e/ou seus subprodutos. A doença tem sido descrita pela ingestão de milho maduro com umidade acima de 15% em temperatura ambiente abaixo de 20°C. O objetivo deste trabalho foi relatar um surto de leucoencefalomacia em equinos alimentados com milho verde. Dois equinos de três animais apresentaram sinais clínicos neurológicos aproximadamente sete dias após iniciarem a ingestão de milho verde na fase reprodutiva (R2, grãos leitosos) com palha e talos, colhido no máximo 24 horas antes de ser administrado. A morte ocorreu aproximadamente 24 horas após o início dos sinais clínicos. Macroscopicamente havia no sistema nervoso central áreas multifocais acinzentadas e amareladas na substância branca do telencéfalo, no hipocampo e no tálamo. Histologicamente observou-se edema e hemorragia em diversas áreas da substância branca do telencéfalo, que correspondiam às áreas acinzentadas observadas na macroscopia. Havia, também, próximo as áreas hemorrágicas, focos de malacia com presença de astrócitos reativos com abundante citoplasma eosinophilico e algumas células inflamatórias. Degeneração das paredes dos capilares e tumefação das células endoteliais também foram observadas. Na análise da amostra de milho pelo método de colunas de imunoafinidade (VICAM) foram detectados 2ppm de fumonisina. A atividade de água neste cereal, quando o grão ainda está leitoso, é de 0,98, o que predispõe ao crescimento de fungos produtores de micotoxinas. No presente caso fumonisina foi encontrada nos grãos leitosos no início da fase reprodutiva (R2), o que sugere que mesmo o milho ainda imaturo pode estar infectado por Fusarium spp. e não deve, também, ser administrado aos equinos.

Effectiveness of the Pilates Method in the Treatment of Chronic Mechanical Neck Pain: A Randomized Controlled Trial.

OBJECTIVE: To assess the effectiveness of the Pilates method on pain, function, quality of life, and consumption of pain medication in patients with mechanical neck pain. DESIGN: The design was a randomized controlled trial, with a blinded assessor and intention-to-treat analysis. SETTING: The study took place in the outpatient clinic of the rheumatology department, referral center. PARTICIPANTS: Patients (N=64) with chronic mechanical neck pain were randomly allocated to 2 groups: the Pilates group (PG) and a control group (CG). INTERVENTIONS: The PG attended 2 sessions of Pilates per week, for 12 weeks. The protocol included Pilates exercises performed on a mat and on equipment and was adapted depending on the physical fitness of each participant; the repetitions varied from 6 to 12, respecting patient reports of fatigue and pain, using a single series for each exercise. The CG received only the standard pharmacological treatment. Both groups were instructed to use acetaminophen 750 mg if necessary. Patients were evaluated at baseline after 45, 90, and 180 days. MAIN OUTCOME MEASURES: We used the Numerical Pain Scale for pain, the Neck Disability Index for function, and the SF-36 questionnaire for quality of life. RESULTS: The groups were homogeneous at baseline, the only exception being body mass index (BMI), with the PG showing higher BMI. Regarding the assessment between groups over time, statistical differences were identified for pain (P<.001), function (P<.001) and the SF-36 (functional capacity, P=.019; pain, P<.001; general health, P=.022; vitality, P<.001; mental health, P=.012) with the PG consistently achieving better results. Drug consumption was lower in PG patients (P=.037). CONCLUSIONS: This trial demonstrated the effectiveness of the Pilates method for the treatment of chronic mechanical neck pain, resulting in improvement of pain, function, quality of life, and reduction of the use of analgesics.

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases / Proteínas de fase aguda no soro e no líquido cefalorraquidiano de bovinos hígidos: possíveis usos na avaliação de doenças neurológicas

Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender.(AU)

O uso de proteínas de fase aguda (PFAs) para a avaliação da saúde e da doença em animais de produção tem aumentado consideravelmente na última década. O objetivo deste estudo foi determinar a concentração normal de PAFs no soro e no líquido cefalorraquidiano (LCR) de bovinos sadios por meio da eletroforese em gel de poliacrilamida. Foram avaliados cinquenta animais mestiços (350±70kg de PV e 18±1,2 meses de idade), 25 novilhas e 25 novilhos. As amostras de LCR foram colhidas no espaço atlanto-occipital (AO) e as amostras de sangue obtidas da veia jugular. As PAFs do soro e do LCR foram determinadas através da eletroforese em gel poliacrilamida. Trinta e sete proteínas com pesos moleculares que variaram entre 7 e 37kDa foram identificadas no LCR de todos os animais, independente do sexo. Estas oito proteínas foram nominalmente identificadas como imunoglobulina A e G, ceruloplasmina, transferrina, albumina, α1-antitripsina, glicoproteína ácida, e haptoglobina. As frações de proteínas presentes no LCR não diferiram entre novilhas e novilhos. No soro de machos e fêmeas, 34 proteínas com pesos moleculares entre 7 e 244 kDa foram identificadas. As proteínas do soro foram similarmente identificadas, entretanto a α1-antitripsina foi identificada somente no LCR. Os valores séricos de glicoproteína ácida e imunoglobulina G foram significativamente mais elevados nas novilhas em comparação aos novilhos. Em conclusão, a determinação das concentrações de proteínas de fase aguda presentes do LCR pode ser útil no diagnóstico e monitoramento da progressão de doenças neurológicas bovinas, talvez possa ainda direcionar procedimentos terapêuticos. O perfil eletroforético do LCR de bovinos hígidos não se altera em função do sexo.(AU)

Augmented Responses to Ozone in Obese Mice Require IL-17A and Gastrin-Releasing Peptide.

Ozone and obesity both increase IL-17A in the lungs. In mice, obesity augments the airway hyperresponsiveness and neutrophil recruitment induced by acute ozone exposure. Therefore, we examined the role of IL-17A in obesity-related increases in the response to ozone observed in obese mice. Lean wild-type and obese db/db mice were pretreated with IL-17A-blocking or isotype antibodies, exposed to air or ozone (2 ppm for 3 h), and evaluated 24 hours later. Microarray analysis of lung tissue gene expression was used to examine the mechanistic basis for effects of anti-IL-17A. Compared with lean mice, ozone-exposed obese mice had greater concentrations of BAL IL-17A and greater numbers of pulmonary IL-17A+ cells. Ozone-induced increases in BAL IL-23 and CCL20, cytokines important for IL-17A+ cell recruitment and activation, were also greater in obese mice. Anti-IL-17A treatment reduced ozone-induced airway hyperresponsiveness toward levels observed in lean mice. Anti-IL-17A treatment also reduced BAL neutrophils in both lean and obese mice, possibly because of reductions in CXCL1. Microarray analysis identified gastrin-releasing peptide (GRP) receptor (Grpr) among those genes that were both elevated in the lungs of obese mice after ozone exposure and reduced after anti-IL-17A treatment. Furthermore, ozone exposure increased BAL GRP to a greater extent in obese than in lean mice, and GRP-neutralizing antibody treatment reduced obesity-related increases in ozone-induced airway hyperresponsiveness and neutrophil recruitment. Our data indicate that IL-17A contributes to augmented responses to ozone in db/db mice. Furthermore, IL-17A appears to act at least in part by inducing expression of Grpr.

IL-33 Drives Augmented Responses to Ozone in Obese Mice.

BACKGROUND: Ozone increases IL-33 in the lungs, and obesity augments the pulmonary effects of acute ozone exposure. OBJECTIVES: We assessed the role of IL-33 in the augmented effects of ozone observed in obese mice. METHODS: Lean wildtype and obese db/db mice were pretreated with antibodies blocking the IL-33 receptor, ST2, and then exposed to ozone (2 ppm for 3 hr). Airway responsiveness was assessed, bronchoalveolar lavage (BAL) was performed, and lung cells harvested for flow cytometry 24 hr later. Effects of ozone were also assessed in obese and lean mice deficient in γδ T cells and their wildtype controls. RESULTS AND DISCUSSION: Ozone caused greater increases in BAL IL-33, neutrophils, and airway responsiveness in obese than lean mice. Anti-ST2 reduced ozone-induced airway hyperresponsiveness and inflammation in obese mice but had no effect in lean mice. Obesity also augmented ozone-induced increases in BAL CXCL1 and IL-6, and in BAL type 2 cytokines, whereas anti-ST2 treatment reduced these cytokines. In obese mice, ozone increased lung IL-13+ innate lymphoid cells type 2 (ILC2) and IL-13+ γδ T cells. Ozone increased ST2+ γδ T cells, indicating that these cells can be targets of IL-33, and γδ T cell deficiency reduced obesity-related increases in the response to ozone, including increases in type 2 cytokines. CONCLUSIONS: Our data indicate that IL-33 contributes to augmented responses to ozone in obese mice. Obesity and ozone also interacted to promote type 2 cytokine production in γδ T cells and ILC2 in the lungs, which may contribute to the observed effects of IL-33. Citation: Mathews JA, Krishnamoorthy N, Kasahara DI, Cho Y, Wurmbrand AP, Ribeiro L, Smith D, Umetsu D, Levy BD, Shore SA. 2017. IL-33 drives augmented responses to ozone in obese mice. Environ Health Perspect 125:246-253; http://dx.doi.org/10.1289/EHP272.

Perfil dos pacientes com deficiências de desenvolvimento sob atendimento odontológico em um projeto de extensão intersetorial / Profile of patients with developmental disabilities under dental care in an intersectoral extension project

Objetivo: Descrever o perfil dos pacientes atendidos em uma instituição filantrópica onde funciona o projeto de extensão "Atendimento Odontológico a Pacientes com Necessidades Especiais". Métodos: A amostra foi composta por 581 prontuários de pacientes com necessidades especiais de 0 a 33 anos de idade. Foram coletadas informações sobre as prevalências de cárie nas dentições decídua e permanente, gengivite, xerostomia, bruxismo, refluxo gastroesofágico, sexo, uso de medicação de ação central, uso de chupeta, sucção digital, alimentação e higiene bucal. Os dados foram analisados por meio da estatística descritiva e teste do qui-quadrado para verificação de associação entre diagnóstico e sexo. Resultados: 51,93% dos pacientes eram meninos, 71,42% possuíam paralisia cerebral, 12,36% tinham refluxo gastroesofágico, 49,30% usavam medicação anticonvulsivante. O diagnóstico de paralisia cerebral quadriespástica esteve associado ao sexo masculino. Dentre as alterações bucais, 4,86% dos casos apresentaram xerostomia. O uso da chupeta foi encontrado em 26,93% dos indivíduos e 18,05% chupavam o dedo. Alterações gengivais foram detectadas em 15,61% das fichas. Em relação à alimentação, 56,97% dos indivíduos consumiam açúcar acima do aceitável, e com relação à higiene bucal, 14,48% foram classificados como ruim e 17,57% moderada. Em 20,57% dos prontuários havia registro de dentes decíduos cariados e em 4,29% de dentes obturados. Do total de pacientes que possuíam dentes permanentes, 13,17% tinha dentes cariados e 7,75% possuía dentes obturados. A prevalência de cárie dentária é moderada com pequena proporção de dentes restaurados. A alimentação é rica em sacarose para quase 60% destes pacientes. Conclusão: os hábitos parafuncionais apresentam um percentual preocupante de prevalência. Por isso, estes pacientes possuem um perfil de doenças bucais com gravidade moderada e que devem ter um acompanhamento odontológico sistemático para que a prevalência de doenças bucais não aumente com o passar dos anos.(AU)

Aim: The present study aimed to describe the profile of the patients who received dental care at a philanthropic institution, within the project "Dental Care for Patients with Special Needs". Methods: This study was carried out by evaluating 581 records of patients with special needs from 0 to 33 years of age. Data were collected on the prevalence of caries in deciduous and permanent dentitions, gingivitis, xerostomia, bruxism, gastroesophageal reflux, sex, use of central action medication, pacifier use, digital suction, feeding, and oral hygiene. Data were analyzed using descriptive statistics and the chi-square test to verify an association between diagnosis and sex. Results: The results showed that 51.93% were boys, 71.42% had cerebral palsy, 12.36% had gastroesophageal reflux, and 49.30% used anticonvulsant medication. The diagnosis of quadrispastic cerebral palsy was associated with males. Xerostomia was observed in 4.86% of the cases. The use of pacifiers was present in 26.93% of the individuals, while 18.05% of the patients used to suck their finger. Gingival changes were detected in 15.61% of the records. In relation to food, 56.97% of the individuals consumed sugar above the acceptable amount, and when oral hygiene was considered, 14.48% were classified as poor and 17.57% as moderate. This study also identified 20.57% of decayed and 4.29% of filled deciduos teeth. Among the patients who had permanent teeth, 13.17% had decayed teeth, while 7.75% had filled teeth. It could therefore be concluded that the prevalence of dental caries is moderate with a small proportion of treated teeth. Diet is rich in sucrose for almost 60% of these patients. Conclusion: The parafunctional habits present a worrisome percentage of prevalence. Therefore, these patients have a profile of oral diseases with moderate severity and should undergo systematic dental follow-up so that the prevalence of oral diseases does not increase over the years.(AU)